There have been several sightings of the bobcat missing from Reflection Riding in Lookout Valley,.
The animal's handler was on the side of Lookout Mountain trying to make contact with Evi.
The bobcat is believed to be between Reflection Riding and Sunset Rock on the side of the mountain.
Officials said it is believed that young people came up by canoe and opened the pen where Evi the bobcat was kept along with a bald eagle.
Federal law enforcement is now involved in the case.
Officials said, "We now believe that on Monday evening, we experienced a forced and illegal entry at Reflection Riding. Presumably, the young people who trespassed on our property paddled in from Lookout Creek and then illegally entered our boardwalk and the native animal area.
"They forced entry into the home of our bald eagle Flora Nooga, a federally protected migratory bird. While the eagle is currently unsettled, she appears to be uninjured.
"Unfortunately, they also forced their way into the home of our bobcat Evi, who is still missing. We now believe Evi fought back against the criminals and was able to escape before she could be stolen.
"Federal law enforcement is now working the case alongside TWRA." We've created a page to keep everyone up to date on Evi.