Ooltewah High School Principal Mark Bean and Assistant Principal Beverly Hollingsworth have been suspended without pay, school officials said.
The matter is under investigation, it was stated.
It involves an incident occurring on school time, sources said.
School officials are looking into whether they engaged in inappropriate conduct.
A closed-door meeting was held with Ooltewah High faculty on Friday afternoon.
Longtime assistant principal Jim Jarvis was in charge of the school following the departure of the other two administrators.
Ms. Hollingsworth heads the school's international baccalaureate program.
Mr. Bean was named Ooltewah High principal in 2009.
A 1976 Brainerd High School graduate, he is a UTC graduate with a master's from Trevecca College. He taught science for 19 years at Ooltewah High. He coached football for 19 years and was head baseball coach for 12 years.
He was assistant principal at Central High for five years prior to moving to Ooltewah High.
Click here for a promotional video for Ooltewah High produced earlier this year.