Officials said the Hixson Walmart was closing on Tuesday at 2 p.m. for cleaning, sanitizing and restocking.
The store is due to reopen on Thursday at 7 a.m.
Officials said the pharmacy would remain open with alternate pickup options while the store is close. The pharmacy number is 870-3267.
The company-initiated program will allow "extra time for a third-party specialist to further sanitize the store."
All unvaccinated associates must still wear face coverings after the store reopens.
Walmart offers vaccinations to both employees and customers.